Shawn, Why ficoll mouse blood, is there a problem with ACK lysing? We have used this extremely well for phenotyping 100-200 mice by peripheral blood every week. Randy Fischer TherImmune Research Corporation 9700 Great Seneca Hwy Rockville, MD 20850 (240) 453-6256 RFischer@therimmune.com > ---------- > From: shawn_jackson > Sent: Friday, March 24, 2000 3:38 PM > To: Cytometry Mailing List > Subject: ficoll of small volumes > > > hello all- > > I am searching for the best way to ficoll small volumes of blood > (i.e., > 100-200 of mouse blood) efficiently during sample prep for flow. > > presently I am diluting the blood and spinning in 15 ml polypropylene > tubes, > but the cell layer can be frighteningly thin. > > can anyone suggest smaller tubes which have worked? our smaller > 12x75mm tubes > are awfully cloudy and hard to see through. > > thanks > > shawn jackson > Harvard Univ > Boston, MA > >
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