Mara,
Since the fluid systems on the Facscan and FacsCaliber are functionally
similar, using the same sheath fluid for both cytometers should be OK.
Ray Lannigan
Tritech Inc.
800-886-7004
www.tritechinc.com
-----Original Message-----
From: Mara Rocchi <roccm@blackie.mri.sari.ac.uk>
To: cyto-inbox
Date: Monday, March 27, 2000 4:16 PM
Subject: sheath fluid for Calibur
>
>Dear Flowers,
>we are in the process to buy a FACSCalibur, and we would like to here your
>suggestion abut the kind of sheath fluid we must/can use. We aren't
planning
>any sorting, maybe some DNA work, lot and lot of phenotyping and
>intracellular staining.
>>From the archives looks like that different people use different
solutions,
>and for different reasons. I have been using sterile saline for FACScan and
>FACStar, and D'PBS for FACSort, but being new to the Calibur world, I would
>like to be sure that those alternatives to the BD sheath fluid are OK.
>
>Thanks a million in advance
>Mara
>
>Mara S.L. Rocchi
>Moredun Research Institute
>Penicuik EH26 0PZ
>Scotland, UK
>e-mail: roccm@mf.mri.sari.ac.uk
>
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