The question on sheath fluid is whether inexpensive water is equivalent to
expensive saline. We use filtered water on a FASCalibur primarily for DNA
analysis. We have not seen any problems due to possible mixing of sample with
sheath. Using Salt has one potential problem. Salt must be cleaned from the
system or it can cause some major fluidic problems, namely blockage (minor and
major). Water is easier and cheaper to use.
Bob
Robert M. Zucker, Ph.D
U.S. Environmental Protection Agency
National Health and Environmental Effects Research Laboratory
2525 NC Highway 54, MD 72
Research Triangle Park, North Carolina, 27709
tel: 919-541-1585 fax 919-541-4017
e-mail: zucker.robert@epa.gov
|--------+----------------------->
| | lannigan@trit|
| | echinc.com |
| | |
| | 03/28/00 |
| | 08:40 AM |
| | Please |
| | respond to |
| | lannigan |
| | |
|--------+----------------------->
>-------------------------------------------------------|
| |
| To: cytometry@flowcyt.cyto.purdue.edu |
| cc: |
| Subject: Re: sheath fluid for Calibur |
>-------------------------------------------------------|
Mara,
Since the fluid systems on the Facscan and FacsCaliber are functionally
similar, using the same sheath fluid for both cytometers should be OK.
Ray Lannigan
Tritech Inc.
800-886-7004
www.tritechinc.com
-----Original Message-----
From: Mara Rocchi <roccm@blackie.mri.sari.ac.uk>
To: cyto-inbox
Date: Monday, March 27, 2000 4:16 PM
Subject: sheath fluid for Calibur
>
>Dear Flowers,
>we are in the process to buy a FACSCalibur, and we would like to here your
>suggestion abut the kind of sheath fluid we must/can use. We aren't
planning
>any sorting, maybe some DNA work, lot and lot of phenotyping and
>intracellular staining.
>>From the archives looks like that different people use different
solutions,
>and for different reasons. I have been using sterile saline for FACScan and
>FACStar, and D'PBS for FACSort, but being new to the Calibur world, I would
>like to be sure that those alternatives to the BD sheath fluid are OK.
>
>Thanks a million in advance
>Mara
>
>Mara S.L. Rocchi
>Moredun Research Institute
>Penicuik EH26 0PZ
>Scotland, UK
>e-mail: roccm@mf.mri.sari.ac.uk
>
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