RE: sheath fluid for Calibur

From: Fischer, Randy (RFischer@therimmune.com)
Date: Tue Mar 28 2000 - 08:20:01 EST


Mara,

I have been very happy with VWR's blood bank saline for my FACSCalibur.

Randy Fischer
TherImmune Research Corporation
9700 Great Seneca Hwy
Rockville, MD 20850
(240) 453-6256
RFischer@therimmune.com

> ----------
> From:		Mara Rocchi
> Reply To:	roccm@blackie.mri.sari.ac.uk
> Sent:		Monday, March 27, 2000 4:36 AM
> To:	Cytometry Mailing List
> Subject:	sheath fluid for Calibur
>
>
> Dear Flowers,
> we are in the process to buy a FACSCalibur, and we would like to here
> your
> suggestion abut the kind of sheath fluid we must/can use. We aren't
> planning
> any sorting, maybe some DNA work, lot and lot of phenotyping and
> intracellular staining.
> From the archives looks like that different people use different
> solutions,
> and for different reasons. I have been using sterile saline for
> FACScan and
> FACStar, and D'PBS for FACSort, but being new to the Calibur world, I
> would
> like to be sure that those alternatives to the BD sheath fluid are OK.
>
> Thanks a million in advance
> Mara
>
> Mara S.L. Rocchi
> Moredun Research Institute
> Penicuik EH26 0PZ
> Scotland, UK
> e-mail: roccm@mf.mri.sari.ac.uk
>
>



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