Maryalice - I QC every bottle of antibody that comes into our lab and I have not experienced the problem that you describe. At least 75% of our antibodies are from BD. I am, obviously, very interested in the responses that you receive. Abby Kelliher Flow Cytometry Lab - Mass General Hospital -----Original Message----- From: Maryalice Stetler-Stevenson [SMTP:stetler@box-s.nih.gov] Sent: Monday, January 10, 2000 9:43 AM To: Cytometry Mailing List Subject: BD antibodies << File: ATT00006.att >> I would like to poll BD antibody users concerning a recent problem we have had. All of our new BD antibodies have a significantly lower fluorescence than the older lots we QC'd them against. Since the old lots were even brighter when first received, this is quite a poor performance. This makes them unacceptable for clinical use. We are quite shocked and have notified BD that all antibodies failed QC ( I will let you all know the outcome of this, we do expect that they will replace them but you will hear if they don't). This first of all points out to all that you really need to QC antibodies, even if only for research purposes. What I would like to know is if others have had the same problem. We are wondering if this is due to poor shipping conditions leading to deterioration or has BD got a major problem in their antibody production. The latter needs to be discovered as soon as possible for everyone's sake. I can't believe that they would get so greedy that they finally diluted them too far. That would be just too unscrupulous. Maryalice Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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