>I would be grateful if you send me a method for >analyzing mitochondrion activity with R123. >__________________________________________________ >Do You Yahoo!? >Talk to your friends online with Yahoo! Messenger. >http://im.yahoo.com Please my Dear friend! Try to avoid Rh123 staining which is often giving artefact of measurement Better use DiC6(3) in between 1nm and 5 nm in order to avoid any cytotoxic effects or quenching; Take care to use contro with FCCP or mClCCP (Sigma) that you will have to calibrate for collapsing the mitochondrial membrane potential depending of you cell culture medium (depending essentially of the serum added). Or use JC-1 red fluorescence in the same range of concentration after having flitrate the solution to discarb all the red aggregate. I you need reference, send me a new mail; Patrice Petit \ | / (o o) \ | / _______________oOo_____oOo_____oOo_ (o o)__oOo________________ Dr. Petit Patrice X. Institut Cochin de Génétique Moléculaire INSERM U129 - CHU Cochin Port-Royal 24, rue du Faubourg Saint-Jacques F-75014 Paris, France. Tel: 33 01 44 41 24 11 Fax: 33 01 44 41 24 21 E-mail: pxpetit@icgm.cochin.inserm.fr
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