Dear Faisal, Regarding your comments about BD antibodies problems, we also realized that Procount antibodies (NAD,CD34 PE and CD45 PerCP) seems to be diluted, CD45 stainning was near first decade and CD34 had a weak staining compared with CD34 antibody from Immunotech (clone 581) using lyse-no-wash. BD antibodies for immunophenotyping panels are working fine. Best regards Marco A. Fernández Head Cytometry Unit University Hospital Germans Trias i Pujol 08916 Badalona (Barcelona) Spain marcoa@ns.hugtip.scs.es http://personal.redestb.es/marcoafdez ---------- >De: "faisal rawas" <frawas@hotmail.com> >Para: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Asunto: BD antibodies >Fecha: miér., 12 ener 2000 14:10 > > > We have noticed the same thing with I meant "PROCOUNT" reagents. We have > noticed that when you look at the CD45 percp and SSC. Most of the cells are > falling in the first decade, in another word they are negative for CD45. The > CD34 PE is also very weak compared when you use 20ul or even 5ul of CD34 > percp from a different bottle. I am not sure if they are diluting the CD34 > so much as to keep the back ground staining very low, since it is suppose to > be a Lyse no wash technique. > When i add 5 ul of CD45 and CD34 in addition to the recomended volume of > TRUCOUNT reagents the staining looks very nice and bright and your CD34 > population stands out away from the rest of the cells. I think if they > increased their concentration of the antibodies people will be able to use > this reagents with crude bone marrow as well. For this reason we stopped > evaluating the TRUCOUNT reagnets, specially when we are paying four times > the list price. I am not sure if somebody else had also a problem with the > APC Calibrite beads.. there is one particular lot number that keeps failing > the FACSComp on our FACSCalibur, and so far I have not heard any response > from BD in Belgium for an explanation. > > Faisal Rawas > Flow Cytometry Lab > King Faisal Hospital > Riyadh, KSA > ______________________________________________________ > Get Your Private, Free Email at http://www.hotmail.com > >
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