MESF and correcting for isotype matched controls

From: Calman Prussin (CPRUSSIN@niaid.nih.gov)
Date: Thu Jan 06 2000 - 11:42:32 EST 

We are quantitating cell associated antigens using standardized beads and
then generating an MESF from the standard curve generated from the beads.
The question has come up whether or not to "correct" the MESF values
obtained from the specific antibody by subtracting the MESF values obtained
for the  isotype matched controls. 

The problem with correcting the values is that it has little effect on the
samples with high density of Ag, but has a large effect on those with low
expression. As such, if we run replicate samples   on the low expressing
samples we find a larger amount of  variation in the corrected MESFs. My
bias is not to use it, as it seems to introduce more "noise" into the
system. Your thoughts?

Thanks!

> _______________________
> Calman Prussin
> Laboratory of Allergic Diseases
> NIAID/ National Institutes of Health
>

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