Last week we had a similar situation on a lymphnode suspension. Our cells were CD 5 and CD 10 negative....We suspected Large Cell Lymfocytic Lymfoma of B type (after examining in microscsope). Tibor Ric Karlstad, Sweden ¤-----Ursprungligt meddelande----- ¤Från: Anja Porwit [mailto:Anja.Porwit@ks.se] ¤Skickat: den 12 december 2000 09:01 ¤Till: Cytometry Mailing List ¤Kopia: cytometry@flowcyt.cyto.purdue.edu ¤Ämne: Re: atypical B-cell population ¤ ¤ ¤ ¤Did you look at the expression of CD5 and CD10 in these cells? Maybe you ¤are looking at mantle zone cells that can have weak SIg expresion? ¤Kind regards ¤Anna ¤ ¤ ¤At 15:51 2000-12-08 -0500, you wrote: ¤> Gating on these larger cells (by light scatter) shows they are ¤>kappa and lambda negative. Initial gating is done on the CD45+ cells by SS ¤>vs CD45-TC. normal, and other times atypical (but not enough to call it ¤>malignant). Are these cells some sort of normal, B-cells ¤with neither ¤>kappa or lambda? Or do they represent a possible pre-malignant state? How ¤>do you (if you have seen this scenario) report this out? Thanks in ¤>advance Andrea Illingworth Dahl-Chase Diagnostic Services Flow Cytometry ¤>Bangor, Maine ¤Anna Porwit ¤Hematopathology Lab. ¤Department of Pathology, Radiumhemmet ¤Karolinska Hospital, 171 76 Stockholm, Sweden ¤Anja.Porwit@ks.se ¤tel.:+46-851774518 ¤fax.:+46-851775843 ¤ ¤
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