Andrea We dont regularly use CD22, but I strongly suspect that the 'brighter' CD20 cells you see would be dimly CD10+ [especially if you use PE conjugate] and CD23- , whereas the dimmer CD20 cells would be CD10- and CD23+. The CD22 surface staining is generally coordinate with the CD23 staining. I see this all the time in lymph nodes, and your immunophenotypic and light scatter description follows a perfect scheme of B cell nodal maturation [CD19+ cells that down regulate CD10 and upregulate CD20, 23 and 22 along with sIg]. I have had no evidence to suggest this is related to any kind of premalignant state. if you permeabilize the 'brighter' CD20 cells you can sometimes discern polyclonal immunoglobulin light chain expression. F Preffer At 03:51 PM 12/8/00 -0500, Andrea Illingworth wrote: > > We have had quite a few lymph node cases which showed a bimodal distribution > of CD20 and CD22. One population of cells shows expected antigen density of > CD20 and CD22. The second population shows brighter CD20 and slightly dimmer > CD22 expression (same CD45 intensity as the normal cells). BAckgating of > this population shows these cells to have increased light scatter. Gating on > these larger cells (by light scatter) shows they are kappa and lambda > negative. > Initial gating is done on the CD45+ cells by SS vs CD45-TC. > Histolopathology is often normal, and other times atypical (but not enough to > call it malignant). > > What are these cells? Are these cells some sort of normal, B-cells with > neither kappa or lambda? Or do they represent a possible pre-malignant state? > How do you (if you have seen this scenario) report this out? > > Thanks in advance > > Andrea Illingworth > Dahl-Chase Diagnostic Services > Flow Cytometry > Bangor, Maine Frederic I. Preffer Department of Pathology Charlestown Navy Yard- 7140 Massachusetts General Hospital East Charlestown, MA 02129 voice [617] 726-7481 fax [617] 724-3164
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