We have had quite a few lymph node cases which showed a bimodal distribution of CD20 and CD22. One population of cells shows expected antigen density of CD20 and CD22. The second population shows brighter CD20 and slightly dimmer CD22 expression (same CD45 intensity as the normal cells). BAckgating of this population shows these cells to have increased light scatter. Gating on these larger cells (by light scatter) shows they are kappa and lambda negative. Initial gating is done on the CD45+ cells by SS vs CD45-TC. Histolopathology is often normal, and other times atypical (but not enough to call it malignant). What are these cells? Are these cells some sort of normal, B-cells with neither kappa or lambda? Or do they represent a possible pre-malignant state? How do you (if you have seen this scenario) report this out? Thanks in advance Andrea Illingworth Dahl-Chase Diagnostic Services Flow Cytometry Bangor, Maine
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