Don't know about an antibody, but I remember the very early work on cell
surface labelling in 1980 to be the use of tagged insulin which might also be
of interest.
LECTIN AND INSULIN BINDING TO CELL-SURFACES OF DIFFERENT LYMPHOMA STRAINS, AS
STUDIED BY FLOW CYTOFLUOROMETRY
MANSKE W, SAUER A, BOHN B
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE
361: (3) 299-300 1980
Would be interesting to find out about Burkard Bohn's whereabouts. Just
listening to a CD of modern Christmas songs it makes me think of his
piano-skills which I still have a lively memory of. He had an excellent talent
to take any Christmas song through its paces to any musical style. So if anyone
knows - please drop me a line.
Should no other opportunity arise I would already like to wish everyone a
peaceful Christmas time and a healthy and happy 2001 and thank the crew at
Purdue for their services to cytometry.
Regards
gerhard.nebe-von-caron@unilever.com
-----Original Message-----
From: Douglas S. Smoot [SMTP:rin0dss@bumed30.med.navy.mil]
Sent: Thursday, December 07, 2000 10:19 PM
To: Cytometry Mailing List
Subject: Insulin Antibodies
I had an investigator come to me asking if I knew of a source for a Mouse
anti-insulin antibody suitable for intracellular staining and analysis by
flow cytometry. He is interested, of course, in conjugated reagents if they
exist and would be interested in finding out also if they cross-react with
other species of Insulin.
Thank you in advance for your help.
Doug
{-----------------------------------------------------------------------}
Douglas Smoot
NIDDK-Navy Transplantation & Autoimmunity Branch
Naval Medical Research Center
AFRRI Building 46, Room 2415
8901 Wisconsin Avenue
Bethesda, MD 20889-5603
voice: 301-295-1843
fax: 301-295-6484
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