Re: Staining in 96 well plates: TC treated or not?

From: Jose A. Stoute (j.a.stoute@worldnet.att.net)
Date: Sat Dec 04 1999 - 06:41:15 EST


I like to use polystyrene simply because the have larger volume capacity for our
washes. Unless you know of a particular brand of polypropylene that has larger
volume capacity.

Regards,

Jose A. Stoute

Art Roberts wrote:

> I routinely use polystyrene Vee-bottom wells for my staining because I
> believe I lose fewer cells than with U-bottom wells.  I wonder if V-bottom
> wells in polypropylene would be even better?  Any opinions on this?  Are
> these plates available?
>
> Art
>
> On Thu, 2 Dec 1999, Larry Arnold wrote:
>
> >
> > John
> >
> > It's not a dumb question.
> > We prefer round bottom polyPROPYLENE.  Better for the cells.
> >
> > Larry Arnold
> >
> > At 08:54 PM 12/1/99 -0500,  John D. Altman wrote:
> > >
> > >This is a dumb question.
> > >
> > >We stain cells in 96 well round bottom polystyrene plates. Does anyone know
> > >if it matters whether we use TC treated plates compared to non-treated
> > >plates?
> > >
> >
> > Larry W. Arnold, Ph.D.
> > Res. Assoc. Prof.
> > Director, Flow Cytometry Facility
> > Department of Microbiology and Immunology
> > CB# 7290
> > University of North Carolina
> > Chapel Hill, NC 27599
> > Phone: 919-966-1530
> > FAX: 919-962-8103
> >
>
> __________________________________
> Arthur Roberts
> Dept. of Medicine
> Robert Wood Johnson Medical School
>
> email:  robertar@umdnj.edu
> phone:  732-235-7790
> Fax:    732-235-7792

--
Jose A. Stoute, MD
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