Hi Chris, in our case the cells were actually lysing as they weren't compatible with the medium being used. I'd also do a drop delay check. The cell number on the instrument counts all the cells that fit in the sort gates but that does not mean it has actually sorted it. regards Ann At 13:35 02.12.99 -0800, you wrote: > >Hello gang, >My question deals with our FacStar. I am getting reports of counts that are >about 1/2 of the cell number the instrument says were recovered. I am >wondering if anyone has seen this or what to do to fix this problem. Cell >count is very important in many of our studies. >Thanks in advance and Happy Holidays, > >chris > > > > > >Chris Lena >Laboratory Technician >La Jolla Institute For Allergy and Immunology >phone:(858)678-4536 >fax:(858)678-4595 >cali@liai.org >http://www.liai.org > > _ _ > (_)-(_) > \"/ > =V= > > > > >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:18 EST