Jim,
I've been looking for a reference for exactly this issue myself, and I look
forward to reading your paper. However, you appear not to have the reference
quite right. For those of you with access to Wiley journals on the web, I
found the paper on the web at
http://www3.interscience.wiley.com/cgi-bin/abstract?ID=48586
The reference is --
Cytometry
Volume 27, Issue 3, 1997. Pages: 233-238.
Thanks again for pointing it out....
John
on 12/2/99 3:13 PM, Leary, James F. at jleary@utmb.edu wrote:
>
> Hai -- You might find our paper on rare cell sampling statistics helpful
> (Rosenblatt, J.I., Hokanson, J.A., McLaughlin, S.R., Leary, J.F.: "A
> Theoretical Basis for Sampling Statistics Appropriate for the Detection and
> Isolation of Rare Cells Using Flow Cytometry and Cell Sorting" Cytometry
> 26: 1-6; 1997). -- Jim Leary
>
> -----Original Message-----
> From: Qi, Hai [mailto:haqi@UTMB.EDU]
> Sent: Wednesday, December 01, 1999 4:49 PM
> To: cyto-inbox
> Subject: rare event
>
>
>
>
> It is really not too rare, a cell population about 0.04% to 0.15%, while the
> background is 0.01-0.05%. The problem is the variation among different
> measurements, presumably due to the systematic error. For example, I had
> 10e7 total cells and ran 2*10e5 out of them for two times: one time I got
> 0.04% and the other time I got 0.15%. In order to have smaller variation,
> obviously I have to increase my sample size, but the last thing I want to do
> is to run through all 10e7 cells. Can anyone give me a handy statistical
> method for estimating the sample size that makes a cost-effective
> compromise? Thank you very much.
>
> Hai Qi
> Dept. of Pathology, UTMB
>
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