Hai -- You might find our paper on rare cell sampling statistics helpful (Rosenblatt, J.I., Hokanson, J.A., McLaughlin, S.R., Leary, J.F.: "A Theoretical Basis for Sampling Statistics Appropriate for the Detection and Isolation of Rare Cells Using Flow Cytometry and Cell Sorting" Cytometry 26: 1-6; 1997). -- Jim Leary -----Original Message----- From: Qi, Hai [mailto:haqi@UTMB.EDU] Sent: Wednesday, December 01, 1999 4:49 PM To: cyto-inbox Subject: rare event It is really not too rare, a cell population about 0.04% to 0.15%, while the background is 0.01-0.05%. The problem is the variation among different measurements, presumably due to the systematic error. For example, I had 10e7 total cells and ran 2*10e5 out of them for two times: one time I got 0.04% and the other time I got 0.15%. In order to have smaller variation, obviously I have to increase my sample size, but the last thing I want to do is to run through all 10e7 cells. Can anyone give me a handy statistical method for estimating the sample size that makes a cost-effective compromise? Thank you very much. Hai Qi Dept. of Pathology, UTMB
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