David.McFarland@mcmail.vanderbilt.edu wrote: > > Good day all. I would like suggestions for a source of sterile, endotoxin free > water or PBS. I have been making my own PBS, but my water source, a lab > filtration system that I'm not in charge of, isn't that great. I have gotten > contamination with algae and bacteria. I can filter and autoclave to sterilize > and remove particles, but I still have the endotoxin problem. Sorting of > lymphocytes for functional assays demands endotoxin free buffer. Anybody else > have this problem? David: Since you are at a medical center, do you have access to a university catalog? Here at UT it is called a "Stores" catalog, or a catalog for the facility that stocks items for the entire institution. In this catalog, we can find pens, pencils, paper, and to the point, 0.9% Normal Saline sold in sterile 1 liter bags from Baxter and is the same stuff used in the hospitals. For us it is fairly cheap when taking into account the reagents, sterilizaiton, containers, and time. Our facility probably isn't' as busy as yours but we've only had one contamination of the Vantage in the last 2.5 years... Hope this helps, David =========== David L. Haviland, Ph.D., Asst. Prof. Immunology University of Texas - Houston, H.S.C. Institute of Molecular Medicine, R907 2121 W. Holcombe Blvd., Houston, TX 77030 713.500.2413-Voice//713.500.2424-FAX ----------------- If everything seems to be going so well, you have obviously overlooked something. ==========
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