When we used multi Q-preps at ARUP, we also had problems with them going down a lot, and also had the problem of having our broken machines replaced with broken machines. Most of the time, it was the C reagent syringe that was faulty. We had a good one that we kept around and used to replace the syringe on the Q-preps that were sent to us. We eventually went to the new T Q-preps, and they were excellent. Coulter went to glass syringes on that machine, and better overall construction. I would suggest taking a look at them. They were worth the larger price tag in my estimation, because they performed virtually flawlessly, and we had used them for almost a year before I left that lab. Dax Arguello Huntsman Cancer Institute Flow Cytometry Core Facility Salt Lake City, UT dax.arguello@hci.utah.edu (801) 581-8641 -----Original Message----- From: Selvaggi, Thomas M.D. [mailto:TSelvaggi@humed.com] Sent: Friday, November 26, 1999 3:21 PM To: cyto-inbox Subject: RE: Calibur & tube spillover on carousel The XL is an excellent clinical machine, although lately we've had difficulty getting our Q prep machine replaced. Think about this also. If you're automated but have to do hand lysis because another part of the process is not supported, isn't the automation purpose defeated? We've actually had our broken Q prep machine replaced by a broken machine! This comes curiously at a time when the company has manufactured a new, more expensive lysis machine. Go with the XL, but keep your eyes WIDE open. -----Original Message----- From: Dax Arguello [mailto:Dax.Arguello@hci.utah.edu] Sent: Monday, November 22, 1999 5:02 PM To: Cytometry Mailing List Subject: RE: Calibur & tube spillover on carousel Hello, There was some discussion about this a while ago. I've used both instruments with carousels, and I much preferred the XL. Spillover was not a problem as it was with the Calibur. The spillover on the Calibur can be solved by adding a wash tube between samples, or increasing the time between tubes. This was a luxury we didn't have. As far as the sheath fluid goes, the tank on the XL is also much larger than the tank on the Calibur. With the XL in a high volume clinical lab, we had to refill the sheath perhaps once a day. The tank on the Calibur needed to be filled 3 or 4 times a day, and sheath is used on the Caliburs even while the SIP is open. Something to think about. Even in a research core facility like I work in now, the BD tanks manage to get filled 3 or 4 times daily. Both are capable machines, but if it's automated sample processing you're looking for, I'd go with Coulter. Dax Arguello Huntsman Cancer Institute Flow Cytometry Core Facility Salt Lake City, UT dax.arguello@hci.utah.edu (801) 581-8641 -----Original Message----- From: Priest, Richard C [mailto:rp15456@GlaxoWellcome.co.uk] Sent: Friday, November 19, 1999 9:17 AM To: cyto-inbox Subject: Calibur & tube spillover on carousel Dear Flowers, I'm interested in getting a Calibur but seem to remember seeing some e-mails a while ago indicating spillover occurred when using the carousel. Can any of you end users give me an idea of how bad a problem this is please. Another concern is that it got through sheath tank fluid by the gallon.. is this a reality ? The other machine we are considering is the Excell which seems to do pretty much the same from what I can see. If anyone has strong opinions for either machine I'd be interested to hear them. Thanks Richard Richard Priest Cellular Biochemistry Glaxo Wellcome R&D Ltd Gunnels Wood Road Stevenage Hertfordshire SG1 2NY UK phone: +44 1438 764094 FAX: +44 1438 764818 email: rp15456@glaxowellcome.co.uk
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