We have an investigator who wants to detect activated T lymphocytes in rats by staining for CD25, the IL 2R alpha chain. We have suggested a better way would be to do two-color staining for CD3 and CD122, IL 2R beta chain. Is there anyone out there doing rat splenic T lymphocytes and what would be your recommendations and MAbs to use? Thanks! ******************************************************** * John H. Wyckoff III, PhD * * Associate Professor of Immunology * * Department of Infectious Diseases and Physiology * * College of Veterinary Medicine * * Oklahoma State University * * Stillwater, Oklahoma 74078-2006 * * telephone: 405-744-8176 * * facsimile: 405-744-8263 * * e-mail: wyckoff@okstate.edu * ******************************************************** \ | / (o o) _________________________oOo_ ( )__oOo________________ "I try to take one day at a time but lately several days have attacked me at once!"
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