An even bigger issue is that the PE-CY5 is chemically synthesized and therefore is not uniform in its energy transfer (or PE leakage as referred to by Carleton Stewart) from manufacturer to manufacturer. This can make compensation a real nightmare (see Communications in Clinical Cytometry vol 38, no 4, p161) PerCP is a naturally occurring compound and does not suffer from this inconsistency. Margaret On 11/28 Michael Ormerod wrote: I have not worked on this system myself but I am told that the He-Ne laser >will excite the Cy5 on the PE-Cy5 conjugate directly, which accounts for >the need to apply so much compensation. Looking at the excitation/emission >spectra, this is what would be expected. > >I think that it might be best to use PerCP as the third dye. > >Michael Ormerod >34 Wray Park Road >Reigate RH2 ODE >Telephone: +44 (0)1737 241726 >PLEASE NOTE NEW FAX & MOBILE NUMBERS >FAX: +44 (0)1737 226736 >Mobile telephone: 07802 293242 >Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> Margaret M. Tropea Critical Care Medicine Department National Institutes of Health (ph) 301-496-7752 (fax) 301-480-3389 >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
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