make a regular RPMI and 40% Fetal Calf Serum mix with 10% DMSO Freeze your pbl's in it and they will be fine for flow. On Thu, 2 Sep 1999, Te Chih Liu wrote: > > >Does anyone have a protocol for freezing whole blood that > >would permit subsequent flow cytometry on the thawed sample? > > Fiebig et al. Cytometry 1997; 29:340-350 describes a simple method that > gives comparable results for lymphocyte analysis to analysis on fresh > blood. > > Te-Chih > > Te-Chih Liu, MRCP(UK), MRCPath > Haematology Division > National University Hospital > Singapore > > Phone: (65)-7725353 > Fax: (65)-7751757 > > Email: liutc@pacific.net.sg >
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