I am doing two color immunofluorescence, and have an antibody that is PE conjugated. PE is not bright enough to visualize alone, so I have to amplify with a secondary. However, I cannot use a goat-anti-mouse, because I am using that on the first antibody. Is there a source for HRP-anti-PE that can be used as a secondary? Less desirable, is HRP-anti FITC. Thanks ron Ronald L. Rabin, M.D. Clinical Associate Cytokine Biology Unit, Laboratory of Clinical Investigation National Institute of Allergy and Infectious Diseases National Institutes of Health Bldg. 10/Rm. 11N228 10 Center Drive MSC 1888 Bethesda, MD 20892-1888 Phone: (301) 402-4910 FAX: (301) 402-0627
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