Re: Freeze whole blood or buffy coats??

From: Te Chih Liu (liutc@pacific.net.sg)
Date: Thu Sep 02 1999 - 03:41:30 EST


>Does anyone have a protocol for freezing whole blood that 
>would permit subsequent flow cytometry on the thawed sample? 

Fiebig et al. Cytometry 1997; 29:340-350 describes a simple method that 
gives comparable results for lymphocyte analysis to analysis on fresh 
blood.

Te-Chih

Te-Chih Liu, MRCP(UK), MRCPath
Haematology Division
National University Hospital
Singapore

Phone: (65)-7725353
Fax: (65)-7751757

Email: liutc@pacific.net.sg



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