I want to thank everyone who responded for their helpful comments, for advice and protocols received. The reference from Te Chih (see below) was extremely helpful and validates freezing WB for subsequent performance of CD4 and CD8 lymphocyte markers. I am interested in monocte markers and just hope that the same will hold. I would also just reiterate what others have said: this is an incredibly active and helpful list. People are very generous with sharing experience and protocols, and I appreciate all the helpful responses I received recently, in response to this and to several other queries. Thanks again, Stan > Subject: Re: Freeze whole blood or buffy coats?? > Date: Thu, 2 Sep 99 08:41:30 -0000 > From: Te Chih Liu <liutc@pacific.net.sg> > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > > >Does anyone have a protocol for freezing whole blood that > >would permit subsequent flow cytometry on the thawed sample? > > Fiebig et al. Cytometry 1997; 29:340-350 describes a simple method that > gives comparable results for lymphocyte analysis to analysis on fresh > blood. > > Te-Chih > > Te-Chih Liu, MRCP(UK), MRCPath > Haematology Division > National University Hospital > Singapore > > Phone: (65)-7725353 > Fax: (65)-7751757 > > Email: liutc@pacific.net.sg > > >
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