John Highly recommend that you use frosted slide ends to make cell suspensions. Much cheaper and better. Must use real frosted (etched) slides not the white painted ones. Larry At 04:01 PM 8/10/99 -0500, you wrote: > > Dear Flowers: > > It has been seemingly eons since my lab did much mouse work. Now we find > ourselves gearing up again for a lot of mouse work with splenocytes. We need > to make more stainless steel screens for mincing spleens and pressing the > fragments through with syringe plungers. The problem is we have lost our > source for the screening. It appears we used 100-200 mesh size. Has anyone > a clue as to a US supplier? Many thanks. > > JHWIII > > ******************************************************** * * > * Department of Infectious Diseases and * * * * * * * > ******************************************************** > > > > \ | / > (o o) > _________________________oOo_ ( )__oOo________________ * John H. > Wyckoff III, > PhD > > * Associate Professor of > Immunology > Physiology > * College of Veterinary > Medicine > > * Oklahoma State > University > > * Stillwater, Oklahoma > 74078-2006 > * telephone: > 405-744-8176 > > * facsimile: > 405-744-8263 > > * e-mail: > wyckoff@okstate.edu > r>"I try to take one day at a time but lately several days > have attacked me at once!" > > > > Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology University of North Carolina Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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