>I was wondering if anyone tried this method to count apoptotic cells by >flow, i.e., using unlabeled Annexin V plus anti-Annexin V mAb-FITC? I think >it would be much cheaper than Annexin-FITC. Let's do some calculations: If you're unwilling to bear the cost of a QC'd reagent from a manufacturer, then you should consider making the FITC annexin yourself. The FITC is going to be MUCH cheaper than anti-annexin V mAb, and, will make your experiment that much easier again (single step vs two step and multiple controls to alleviate worries about expression of AxV protein). To see how easy it is to conjugate FITC to Annexin V (purified), see <http://www.drmr.com/abcon/AxVFITC.html>. mr
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