This is a question to the clinical users doing immuno-phenotyping in the group. Has anyone got a comment to make on the differing phenotypes of mononuclear cells that are sometimes seen when the same sample is prepared using a lysed whole blood approach as opposed to PBMC obtained after a ficoll separation. What about marrow samples? I'm presently looking at a sample from a 6-year old child with CD10+ ALL. On the ficoll preparation, the plots are that of a straight forward common ALL. On the LWB preparation, there is a distinct CD5+/CD20+ population (15% of MNC), separate from the CD5-/CD20+ B-cells/blasts and the residual CD5+/CD20- T-cells. I can't think of any reason why that population should be there at all. Thanks. Te-Chih Liu Te-Chih Liu, MRCP(UK), MRCPath Haematology Division National University Hospital Singapore Phone: (65)-7725353 Fax: (65)-7751757 Email: liutc@pacific.net.sg
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