Hello flow people I would like to ask you which would be the most appropriate (or the most result-promising) methodology for single nuclei/cells preparation from paraffin embedded specimens of soft tissue sarcomas. Does anyone have some experience in preparing such samples and do they differ somewhat from the other tumors particularly in results of phases assesment (to cut the long story short, what should I expect to obtain?). Please, consider the situation that I didn't have expirienced, yet DNA content analysis from paraffin embedded tissues. I would appreciate any information as well as some references!!! Thanks in advance ViktorViktor Jovic, MD INSTITUTE FOR ONCOLOGY AND RADIOLOGY OF SERBIA Experimental laboratory Dpt. for pathology and Cytology Pasterova 14, 11000 Belgrade Yugoslavia Contacts: Phone: +381 11 685755 ext 426 FAX: +381 11 685300 Mail: vjovic@beotel.yu zvecko@drenik.net www.ncrc.ac.yu
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