This reported at a meeting of, I believe, the Clinical Immunology Society. Ficoll Hypaque decreased B cell percentage to a mean of 35%, but there can be selective depletion of T and NK populations as well. The fact that you see CD5+ B cells may simply reflect the predominance of this type of B cell in children. The reference is Brown, M.R., Cleary, J. M.,and Fleisher, T.A. Selective lyphocyte depletion by ficoll hypaque density gradient centrifugation. I am not sure what meeting this was, or whether this was ever published in a peer reviewed journal. ron Ronald L. Rabin, M.D. Clinical Associate Cytokine Biology Unit, Laboratory of Clinical Investigation National Institute of Allergy and Infectious Diseases National Institutes of Health Bldg. 10/Rm. 11N228 10 Center Drive MSC 1888 Bethesda, MD 20892-1888 Phone: (301) 402-4910 FAX: (301) 402-0627 > ---------- > From: Te Chih Liu > Sent: Monday, August 2, 1999 12:37 PM > To: Cytometry Mailing List > Subject: ficoll vs lysed blood phenotypes > > > This is a question to the clinical users doing immuno-phenotyping in the > group. > > Has anyone got a comment to make on the differing phenotypes of > mononuclear cells that are sometimes seen when the same sample is > prepared using a lysed whole blood approach as opposed to PBMC obtained > after a ficoll separation. > > What about marrow samples? > > I'm presently looking at a sample from a 6-year old child with CD10+ ALL. > On the ficoll preparation, the plots are that of a straight forward > common ALL. On the LWB preparation, there is a distinct CD5+/CD20+ > population (15% of MNC), separate from the CD5-/CD20+ B-cells/blasts and > the residual CD5+/CD20- T-cells. I can't think of any reason why that > population should be there at all. > > Thanks. > > Te-Chih Liu > > > > Te-Chih Liu, MRCP(UK), MRCPath > Haematology Division > National University Hospital > Singapore > > Phone: (65)-7725353 > Fax: (65)-7751757 > > Email: liutc@pacific.net.sg >
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