Of course you can use kappa/lambda on whole blood - just need to wash the blood in PBS. It's that simple. AND, you must use kappa/lambda in combination with CD19. We all had to learn at some point. EP Van Bockstaele, Dirk wrote: > Hi Sebastian, > Never use anti-kappa, lambda reagents on whole blood!!! The ab binds to the > serum Ig that is abundantly present, so there's nothing left over to bind on > the B-cells surface Ig. Not long in the business I guess? > Best Regards, > Dirk > > Prof. Dirk Van Bockstaele, PhD > Laboratory of Hematology > Antwerp University Hospital > Wilrijkstraat 10 > B-2650 Edegem > Belgium > tel. 32 3 821 3900, fax 32 3 825 1148, > e-mail dirk.van.bockstaele@uza.uia.ac.be > > > ---------- > > Van: Sebastian, J, Gelderbloem, Mr[SMTP:sjg@samiot.uct.ac.za] > > Verzonden: woensdag 14 juli 1999 19:09 > > Aan: Cytometry Mailing List > > Onderwerp: Re: CLL PHENOTYPE > > > > > > Dear Flowers > > > > I have a slight problem and I am not sure what the solution is. > > > > We use polyclonal Kappa and Lambda from BD for our chronic > > leukaemia panel. We have been setting the samples up using whole > > blood and for some or other reason the Light Chains are coming up > > negative everytime. We only bitmap lymphoid cells. > > > > Is there anyone with some advice. > > > > Thanks > > Sebastian > >
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