Hi Sebastian, Never use anti-kappa, lambda reagents on whole blood!!! The ab binds to the serum Ig that is abundantly present, so there's nothing left over to bind on the B-cells surface Ig. Not long in the business I guess? Best Regards, Dirk Prof. Dirk Van Bockstaele, PhD Laboratory of Hematology Antwerp University Hospital Wilrijkstraat 10 B-2650 Edegem Belgium tel. 32 3 821 3900, fax 32 3 825 1148, e-mail dirk.van.bockstaele@uza.uia.ac.be > ---------- > Van: Sebastian, J, Gelderbloem, Mr[SMTP:sjg@samiot.uct.ac.za] > Verzonden: woensdag 14 juli 1999 19:09 > Aan: Cytometry Mailing List > Onderwerp: Re: CLL PHENOTYPE > > > Dear Flowers > > I have a slight problem and I am not sure what the solution is. > > We use polyclonal Kappa and Lambda from BD for our chronic > leukaemia panel. We have been setting the samples up using whole > blood and for some or other reason the Light Chains are coming up > negative everytime. We only bitmap lymphoid cells. > > Is there anyone with some advice. > > Thanks > Sebastian >
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