Of course, that is exactly what I meant! I couldn't agree with you more. Dirk > ---------- > Van: Elisabeth Paietta[SMTP:epaietta@chcn.org] > Verzonden: vrijdag 16 juli 1999 16:30 > Aan: Cytometry Mailing List > Onderwerp: Re: CLL PHENOTYPE > > > Of course you can use kappa/lambda on whole blood - just need to wash the > blood > in PBS. It's that simple. AND, you must use kappa/lambda in combination > with > CD19. > We all had to learn at some point. > EP > > > Van Bockstaele, Dirk wrote: > > > Hi Sebastian, > > Never use anti-kappa, lambda reagents on whole blood!!! The ab binds to > the > > serum Ig that is abundantly present, so there's nothing left over to > bind on > > the B-cells surface Ig. Not long in the business I guess? > > Best Regards, > > Dirk > > > > Prof. Dirk Van Bockstaele, PhD > > Laboratory of Hematology > > Antwerp University Hospital > > Wilrijkstraat 10 > > B-2650 Edegem > > Belgium > > tel. 32 3 821 3900, fax 32 3 825 1148, > > e-mail dirk.van.bockstaele@uza.uia.ac.be > > > > > ---------- > > > Van: Sebastian, J, Gelderbloem, Mr[SMTP:sjg@samiot.uct.ac.za] > > > Verzonden: woensdag 14 juli 1999 19:09 > > > Aan: Cytometry Mailing List > > > Onderwerp: Re: CLL PHENOTYPE > > > > > > > > > Dear Flowers > > > > > > I have a slight problem and I am not sure what the solution is. > > > > > > We use polyclonal Kappa and Lambda from BD for our chronic > > > leukaemia panel. We have been setting the samples up using whole > > > blood and for some or other reason the Light Chains are coming up > > > negative everytime. We only bitmap lymphoid cells. > > > > > > Is there anyone with some advice. > > > > > > Thanks > > > Sebastian > > > >
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