Thank goodness for all of the people who took the time to answer all of my "dumb" questions when I was just new to flow. What ever happened to the addage that "the only dumb question is the one that is never asked". I heartly agree with Elizabeth! >Of course you can use kappa/lambda on whole blood - just need to wash the >blood >in PBS. It's that simple. AND, you must use kappa/lambda in combination with >CD19. >We all had to learn at some point. >EP > > >Van Bockstaele, Dirk wrote: > >> Hi Sebastian, >> Never use anti-kappa, lambda reagents on whole blood!!! The ab binds to the >> serum Ig that is abundantly present, so there's nothing left over to bind on >> the B-cells surface Ig. Not long in the business I guess? >> Best Regards, >> Dirk >> >> Prof. Dirk Van Bockstaele, PhD >> Laboratory of Hematology >> Antwerp University Hospital >> Wilrijkstraat 10 >> B-2650 Edegem >> Belgium >> tel. 32 3 821 3900, fax 32 3 825 1148, >> e-mail dirk.van.bockstaele@uza.uia.ac.be >> >> > ---------- >> > Van: Sebastian, J, Gelderbloem, Mr[SMTP:sjg@samiot.uct.ac.za] >> > Verzonden: woensdag 14 juli 1999 19:09 >> > Aan: Cytometry Mailing List >> > Onderwerp: Re: CLL PHENOTYPE >> > >> > >> > Dear Flowers >> > >> > I have a slight problem and I am not sure what the solution is. >> > >> > We use polyclonal Kappa and Lambda from BD for our chronic >> > leukaemia panel. We have been setting the samples up using whole >> > blood and for some or other reason the Light Chains are coming up >> > negative everytime. We only bitmap lymphoid cells. >> > >> > Is there anyone with some advice. >> > >> > Thanks >> > Sebastian >> > >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> Margaret M. Tropea Critical Care Medicine Department National Institutes of Health (ph) 301-496-7752 (fax) 301-480-3389 >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
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