Doug, I can answer part of your question: >If one gives IL-2 to T-cells they should proliferate (?), perhaps >activated cells will start first/go faster than others (?) naive T cells will not proliferate or upregulate CD69 in response to IL-2 alone. They need a signal through TCR (and optimally, through CD28 as well). However, primed T cells, especially recently (~7 days) primed cells, express high affinity IL-2R and still 'remember' that they received a signal through their TCR awhile back, and will proliferate in response to IL-2 alone. > do they up-regulate CD38, or more importantly CD69 in response to the >cytokine alone? Unfortunately, I'm not sure whether these primed T cells upregulate CD69 to IL-2 alone. The current dogma (as you probably know) is that signals eminating from TCR are necessary and sufficient for the upregulation of CD69 - but I have had a difficult time finding instances in the literature where this has been strictly tested (i.e., can cytokines alone cause CD69 upregulation, especially when the cytokine has some other biological effect consistent with activation). So hopefully someone out there can add some insight to this question. AW Andrew D. Wells, Ph.D. University of Pennsylvania Department of Medicine 904 Stellar-Chance Laboratories 422 Curie Boulevard Philadelphia, PA 19104 (215) 898-1951 (215) 573-2880 (FAX) adwells@mail.med.upenn.edu
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