Hello David, You might want to check references by H.-R. Rodewald- he has done quite a bit of work detecting and tracking cells after injection in mice. As a start, check out Cell, Vol. 69, 139-150 in the April 3, 1992. Peter Lopez > ---------- > From: > David.McFarland@mcmail.vanderbilt.edu[SMTP:David.McFarland@mcmail.vand > erbilt.edu] > Sent: Wednesday, July 07, 1999 2:20 PM > To: Cytometry Mailing List > Subject: rare event analysis > > > > > I have started running samples for a research project where the > investigators > label mouse lymphs with either CFSE or transfect with GFP and then > transplant > into another mouse. After 1 to 7 days they remove tissues from the > recipient > mouse (blood, spleen, thymus, lymph node) and try to detect the donor > cells. > These events can be very rare (<1%). They are also labelling two > other surface > markers, so it is three color staining. Any tips on doing this right > would be > greatly appreciated. I am most concerned about the number of events > to collect > and what to use for a positive control for CFSE/GFP for compensation. > Thanks in > advance. > > David McFarland > Howard Hughes Medical Institute > Flow Cytometry Facility > Vanderbilt University Medical Center > >
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