A quick comment to the last point. I found that treatment with IFN-alpha/beta alone did activate CD69 on NK and CD8 cells (after only four hours) Artur > -----Original Message----- > From: Andrew D. Wells, Ph.D. [SMTP:adwells@mail.MED.UPENN.EDU] > Sent: Thursday, July 08, 1999 10:27 AM > To: Cytometry Mailing List > Subject: Re: Activation > > > Doug, > I can answer part of your question: > > >If one gives IL-2 to T-cells they should proliferate (?), perhaps > >activated cells will start first/go faster than others (?) > > naive T cells will not proliferate or upregulate CD69 in response to IL-2 > alone. They need a signal through TCR (and optimally, through CD28 as > well). However, primed T cells, especially recently (~7 days) primed > cells, express high affinity IL-2R and still 'remember' that they received > a signal through their TCR awhile back, and will proliferate in response > to > IL-2 alone. > > > do they up-regulate CD38, or more importantly CD69 in response to the > >cytokine alone? > > Unfortunately, I'm not sure whether these primed T cells upregulate CD69 > to > IL-2 alone. The current dogma (as you probably know) is that signals > eminating from TCR are necessary and sufficient for the upregulation of > CD69 - but I have had a difficult time finding instances in the literature > where this has been strictly tested (i.e., can cytokines alone cause CD69 > upregulation, especially when the cytokine has some other biological > effect > consistent with activation). So hopefully someone out there can add some > insight to this question. > > AW > > > Andrew D. Wells, Ph.D. > University of Pennsylvania > Department of Medicine > 904 Stellar-Chance Laboratories > 422 Curie Boulevard > Philadelphia, PA 19104 > (215) 898-1951 > (215) 573-2880 (FAX) > adwells@mail.med.upenn.edu >
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