Hi Janet, There are a number of companies out there who make conjugated Annexins. I too have a lot of users using GFP-tagged cells where we need to get away from FITC. I use a PE-conjugated Annexin from Pharmingen. They also do a biotinylated version as do Oncogene. Caltag market an APC-conjugated version - and I am sure that there are others out there as well. Presumably though you would also want to add a DNA dye for live/dead exclusion, so a PE version would allow you to use 7AAD on a single laser machine or TO-PRO-3 on a dual laser Calibur. Hope that helps! Derek On Fri, 9 Jul 1999, Janet E. Lewis wrote: > We have a user who wants to use annexin to detect apoptosis in > GFP-transfected cells. Does anyone know where one could get annexin > conjugated to something that's not green but still excited by 488nm, PE for > example? ************************************************************************ Derek Davies Voice: (44) 0171 269 3394 FACS Laboratory, FAX: (44) 0171 269 3100 Imperial Cancer Research Fund, e_mail: derek.davies@icrf.icnet.uk London, UK Web Page: http://www.icnet.uk/axp/facs/davies/index.html In tenebris lux *************************************************************************
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