The BD CD69 clone is excellent. The FITC is a bit dull for my taste, but the PE and APC should work well for you. Don't forget to titer it, as the concentration used for cell surface staining may be somewhat different from that used after PFA fixation (and you may end up saving some $$ [or DM] as well). > _______________________ > Calman Prussin > Laboratory of Allergic Diseases > NIAID/ National Institutes of Health > > > ---------- > From: Gregor Rothe > Sent: Tuesday, June 15, 1999 05:00 > To: Cytometry Mailing List > Subject: Intracellular antigens > > > Hello to all, > > In the analysis of T-cell activation we are interested in the > intracellular analysis of activation antigens such as CD25 and > CD69. The intracellular detection of these antigens unfortunately > seems to be highly dependent on the resistence of specific > epitopes to fixation. My question, therefore, would be whether > anyone has experiences in cross evaluating different clones for > CD25 and CD69 regarding effects of paraformaldehyde. > > Thanks for any help > > Gregor Rothe > > *********************************************************** > Institute for Clinical Chemistry and Laboratory Medicine > University of Regensburg > Franz-Josef-Strauss-Allee 11 > D-93053 Regensburg, Germany > > Tel. +49 (941) 944-6204 > Fax +49 (941) 944-6202 > > E-mail: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE > *********************************************************** >
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