Hello to all, In the analysis of T-cell activation we are interested in the intracellular analysis of activation antigens such as CD25 and CD69. The intracellular detection of these antigens unfortunately seems to be highly dependent on the resistence of specific epitopes to fixation. My question, therefore, would be whether anyone has experiences in cross evaluating different clones for CD25 and CD69 regarding effects of paraformaldehyde. Thanks for any help Gregor Rothe *********************************************************** Institute for Clinical Chemistry and Laboratory Medicine University of Regensburg Franz-Josef-Strauss-Allee 11 D-93053 Regensburg, Germany Tel. +49 (941) 944-6204 Fax +49 (941) 944-6202 E-mail: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE ***********************************************************
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