Fluo4/Fura Red

From: David.McFarland@mcmail.vanderbilt.edu
Date: Mon Jun 14 1999 - 13:24:13 EST

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I thank you for the replies to the Fluo4/Fura Red question I posted.  The main
problem was that I was trying to use a log scale where I should be using linear.
Gimme a break, I hadn't done a Calcium Flux assay in about 2 years.  OK, so my
problem went away, but why?  When measuring Fluo4 in FL1 and Fura Red in FL3
compensation (a lot) was needed in both directions.  I run using linear scales
and I don't need any compensation.  Why is that?

David McFarland
Howard Hughes Medical Institute
Flow Cytometry Facility
Vanderbilt University Medical Center

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