--- On Mon, 26 Apr 1999 10:54:05 -0400 Elaine Kunze <mek4@psu.edu> wrote: > >One of my faculty came back from a meeting saying "All the immunologist do >a tetramer assay for antigen presenting cells." Can >anyone provide a reference and or explanation? Peptide/MHC tetrameric complexes are engineered molecules which will specificaaly bind to antigen specific cells and thus are capable of detecting these cells. For example, gag specific tetramers will tag those cells in HIV infected persons which specifically see the gag antigen. Most are MHC I restricted so will label the cytotoxic cells. The ability to resolve the very small percentage of antigen speicific cells is an area of great interest. For a reference try: Altman et al., Science 274: 94, 1996. Regards, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] fax: 516-562-2866 4/28/99 2:59:45 PM E-mail: thomasm@nshs.edu --------------------------------------------------------
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