Elaine,
Please see ref: Science; Vol.274, 4, October 1996. This procedure is described
by John Altman et al. The tetramers, because they and made with PE, are
actually very bright but because of the frequency you will need to collect at
least 100,000 events. I believe the NIH repository is offering samples for
analysis which we are currently using. Please see...www.niaid.nih.gov/repost/
tetramer/listing
Stephen P. Perfetto, MS.,MT. (ASCP)
Walter Reed Army Institute of Research
Department of Molecular Diagnostics and Pathogenesis
1600 East Gude Drive
Rockville, MD. 20850
_______________________________________________________________________________
Subject: tetramer assay??
From: Elaine Kunze <mek4@psu.edu> at Internet_Gateway
Date: 4/26/99 10:54 AM
One of my faculty came back from a meeting saying "All the immunologist do
a tetramer assay for antigen presenting cells."
I feel silly but I never heard of this and didn't find anything after a
quicky look at Current protocols and a few other generic sources. Can
anyone provide a reference and or explanation?
****************************************************************************
Elaine Kunze
Flow Cytometry.....Image Analysis...
The Biotechnology Institute for Research and Education
Life Sciences Consortium
8B Althouse Laboratory (814-863-2762)
Penn State University
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