Dear All, Yes I know this is a flow cytometry database - I am a cytometerist as well - but we all work on proteins and I am looking for help with immunoprecipitating the CDKI, p27. If anyone can get this to work I will be really pleased to hear from you. We seem to have a problem dissociating the protein after immunoprecipitation from the agarose bead such that we get no protein yield.We use 0.5% mercaptoethanol + SDS as the loading buffer to do this. Thanks in advance for your help. Best wishes, John.
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