At 10:54 AM -0400 4/26/99, Elaine Kunze wrote:
>One of my faculty came back from a meeting saying "All the immunologist do
>a tetramer assay for antigen presenting cells."
>
>I feel silly but I never heard of this and didn't find anything after a
>quicky look at Current protocols and a few other generic sources. Can
>anyone provide a reference and or explanation?
>****************************************************************************
> Elaine Kunze
> Flow Cytometry.....Image Analysis...
> The Biotechnology Institute for Research and Education
> Life Sciences Consortium
> 8B Althouse Laboratory (814-863-2762)
> Penn State University
We developed the tetramer assay for analysis of antigen-specific T cell
responses (not APC). There are a number of papers out there on it that are
listed below (some from my lab, some from other labs as well). The first
paper in the list was the "birth announcement" of the MHC tetramers.
You might also want to take a look at the following web site:
http://www.niaid.nih.gov/reposit/tetramer/index.html
John Altman
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1) Altman, J. D., Moss, P. A. H., Goulder, P. J. R., Barouch, D. H.,
McHeyzer-Williams, M. G., Bell, J. I., McMichael, A. J. & Davis, M. M.
(1996). "Phenotypic analysis of antigen-specific T lymphocytes." Science,
274, 94-96.
2) Bousso, P., Casrouge, A., Altman, J. D., Haury, M., Kanelopoulos,
J., Abastado, J.-P. & Kourilsky, P. (1998). "Individual variations in the
murine T cell response to a specific peptide reflect variability in naive
repertoires." Immunity, 9, 169-178.
3) Braud, V. M., Allan, D. S., O'Callaghan, C. A., Soderstrom, K.,
D'Andrea, A., Ogg, G. S., Lazetic, S., Young, N. T., Bell, J. I., Phillips,
J. H., Lanier, L. L. & McMichael, A. J. (1998). "HLA-E binds to natural
killer cell receptors CD94/NKG2A, B and C [see comments]." Nature, 391,
795-9.
4) Busch, D. H., Pilip, I. M., Vijh, S. & Pamer, E. G. (1998).
"Coordinate regulation of complex T cell populations responding to
bacterial infection." Immunity, 8, 353-62.
5) Busch, D. H., Pilip, I. & Pamer, E. G. (1998). "Evolution of a
Complex T Cell Receptor Repertoire during Primary and Recall Bacterial
Infection." J. Exp. Med., 188, 61-70.
6) Callan, M. F., Tan, L., Annels, N., Ogg, G. S., Wilson, J. D.,
O'Callaghan, C. A., Steven, N., McMichael, A. J. & Rickinson, A. B.
(1998). "Direct visualization of antigen-specific CD8+ T cells during the
primary immune response to Epstein-Barr virus In vivo." J. Exp. Med., 187,
1395-402.
7) Crawford, F., Kozono, H., White, J., Marrack, P. & Kappler, J.
(1998). "Detection of antigen-specific T cells with multivalent soluble
class II MHC covalent peptide complexes." Immunity, 8, 675-82.
8) Doherty, P. C. (1998). "The Numbers Game for Virus-Specific CD8+ T
Cells." Science, 280, 227.
9) Dunbar, P. R., Ogg, G. S., Chen, J., Rust, N., van der Bruggen, P.
& Cerundolo, V. (1998). "Direct isolation, phenotyping and cloning of
low-frequency antigen- specific cytotoxic T lymphocytes from peripheral
blood." Curr Biol, 8, 413-6.
10) Flynn, K. J., Belz, G. T., Altman, J. D., Ahmed, R., Woodland, D.
L. & Doherty, P. C. (1998). "Virus-specific CD8+ T cells in primary and
secondary influenza pneumonia." Immunity, 8, 683-691.
11) Gallimore, A., Glithero, A., Godkin, A., Tissot, A. C., Pluckthun,
A., Elliott, T., Hengartner, H. & Zinkernagel, R. (1998). "Induction and
exhaustion of lymphocytic choriomeningitis virus-specific cytotoxic T
lymphocytes visualized using soluble tetrameric major histocompatibility
complex class I-peptide complexes." J. Exp. Med., 187, 1383-93.
12) Gutgemann, I., Fahrer, A. M., Altman, J. D., Davis, M. M. & Chien,
Y. H. (1998). "Induction of rapid T cell activation and tolerance by
systemic presentation of an orally administered antigen." Immunity, 8,
667-73.
13) Heilman, C. A. & Baltimore, D. (1998). "HIV vaccines--where are we
going?" Nat Med, 4, 532-4.
14) Kuroda, M. J., Schmitz, J. E., Barouch, D. H., Craiu, A., Allen, T.
M., Sette, A., Watkins, D. I., Forman, M. A. & Letvin, N. L. (1998).
"Analysis of Gag-specific cytotoxic T lymphocytes in simian
immunodeficiency virus-infected rhesus monkeys by cell staining with a
tetrameric major histocompatibility complex class I-peptide complex." J.
Exp. Med., 187, 1373-81.
15) McHeyzer-Williams, M. G., Altman, J. D. & Davis, M. M. (1996).
"Enumeration and characterization of memory cells in the TH compartment."
Immunol. Rev., 150, 5-21.
16) Murali-Krishna, K., Altman, J. D., Suresh, M., Sourdive, D. J. D.,
Zajac, A. J., Miller, J. D., Slansky, J. & Ahmed, R. (1998). "Counting
Antigen-Specific CD8 T Cells: A Reevaluation of Bystander Activation during
Viral Infection." Immunity, 8, 177-187.
17) Ogg, G. S., et al. (1998). "Quantitation of HIV-1-specific
cytotoxic T lymphocytes and plasma load of viral RNA." Science, 279, 2103-6.
18) Ogg, G. S. & McMichael, A. J. (1998). "HLA-peptide tetrameric
complexes [In Process Citation]." Curr Opin Immunol, 10, 393-6.
19) Sourdive, D. J. D., Murlai-Krishna, K., Altman, J. D., Zajac, A.
J., Whitmire, J., Pannetier, C., Kourilsky, P., Evavold, B., Sette, A. &
Ahmed, R. (1998). "Conserved TCR repertoire in primary and memory CD8 T
cell responses to an acute viral infection." J. Exp. Med., 188, 71-82.
20) Wilson, J. D. K., Ogg, G. S., Allen, R. L., Goulder, P. J. R.,
Kelleher, A., Sewell, A. K., O'Callaghan, C. A., Rowland-Jones, S. L.,
Callan, M. F. C. & McMichael, A. J. (1998). "Oligoclonal expansions of
CD8(+) T cells in chronic HIV infection are antigen specific [In Process
Citation]." J Exp Med, 188, 785-90.
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Dr. John Altman Office: (404) 727-5981
Emory University FAX: (404) 727-3659
Department of Microbiology
and Immunology email: altman@microbio.emory.edu
Emory Vaccine Center
3119 Rollins Research Center
1510 Clifton Rd.
Atlanta, GA 30322
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