--- On Tue, 27 Apr 1999 09:14:25 +0200 Stefanie =?iso-8859-1?Q?Reu=DF?= <reuss@mailer.uni-marburg.de> wrote: >I am writing for the first time and would be interested in your experience >about how few cells are necessary to get decent results. Our lowest number >right now is 5000 per tube. Is there anyone who tries lower numbers? Current ACTG guidelines suggest the following: optimally, 2,500 anchor marker positive cells [i.e. gated cells] minimally, 1,000 anchor marker positive cells For major lymphocyte subsets, it has been reported that 500 cells will provide comparable resutls: Lape'-Nixon and Prince, Cytometry, 26: 223, 1996 Regards, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] fax: 516-562-2866 4/28/99 3:05:48 PM E-mail: thomasm@nshs.edu --------------------------------------------------------
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