One of our grad students is working with transgenic mice that have a construct for beta-galactosidase. She has been detecting the beta-gal with an FDG substrate introduced into the cells ( harvested from newborn mouse thymus and spleen) with hypotonic shock. This method, however, results in cell loss and cell damage and gives high levels of non-specific fluorescence on the flow. We would like to try some other detection method - does anybody know of an antibody to beta-gal that works in flow cytometry? Barbara J Taylor Stem Cell Lab Children's National Medical Center Washington DC
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