Hi The standard red cell lysing solution for human cells is the ammonium chloride lysing solution. We make up a 10X stock and dilute as required. The recipe to make 1 liter of 10X stock is as follows: 80.2g NH4Cl 8.4g NaHCO3 1mM EDTA adjust pH to 7.4 add dH20 to 1 liter We use it to lyse whole blood, adding about 10 times the volume of solution as blood and incubate for about 10-15 minutes on ice. Others use it room temp or at 37 C. I understand red cells are harder to lyse if they have been stored at 4 degrees C. Immature red cells are also hard to lyse. I'll package some up and call it LyseMaster or TurboLyse and sell it you for 100bucks for 10mls if it would make you feel better! Simon Monard ADARC NYC
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