Thanks to all the gurus who have given us food for thought and we have had a lot of fun playing with different analysis tools. The postb doc I am doing the work for is quite new to Flow but very willing to know the how, why and wherefore of what happens. I think she has jumped in the deep end, but is finding the conversation back and forth fascinating For the information of the list the sample was fresh mouse fibroblasts, the results obtained for S-phase were by Brdu Pos analysed in Cell Quest 13.57% analysed in Flowjo 12.94% S-phase by cell cycle analysis in FlowJo (watson pragmatic) 20.94% MacCycle 14.1 % ModFit 23.71%. Make of this what you will. Pick a method and stick to it I guess is what one has to do. Thanks again for all the help and information. Jill Jill
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