Marty, I think that there is a bit of a misunderstanding here with your message. We too have used CCF2 successfully as a substrate for reporter gene assays. It is our understanding however that CCF2 is a substrate for Beta-lactamase not beta-galactosidase. Phil Marder Lilly Research --- BIGOS@Beadle.stanford.edu wrote: > > Barbara- > > You might try the ccf2 substrate system reported in Jan > 1998 Science (Zlokarnik et al). It does not require > hypotonic shock to load, and the emission wavelength is > dependent upon clevage by beta-gal, giving an internal > control for loading. > > It has been used in our facility with good success. It > excites optimally in the low 400 nm range, ideal for a > violet-enhanced krypton laser, but may work as well with > the uv lines of an argon laser. > > -Marty Bigos > Stanford Shared FACS Facility > > > _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:19 EST