Dear Phil, There is a year-old paper that may start you off with a protocol for labelling a virus directly: Leopold. P.L. et al, Human Gene Therapy, 9:367-378; February 10. 1998 " Fluorescent Virions: Dynamic Tracking of the Pathway of Adenoviral Gene Transfer Vectors in Living Cells". This group labelled adenovirus directly and used Cy3 as the fluorochrome. You may not prefer Cy3, but you may want to substitute some small molecule of your preference. According to the authors, the adenovirus retained greater than ninety percent of its titer. This is the key issue. I don't know what virus you are using; some viruses may be more susceptible than others to labelling protocols. The authors apparently had to optimize their dye-to-protein ratios to achieve well-labelled yet biologically active virus particles. I hope this helps! Cheers, Linda Weaver GTI/Systemix Gaithersburg, MD 20878
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